Table 4.

Agreement in seroprevalence results

	Seroprevalence			Cohen’s kappa (95% CI)
Antigen	Run A N = 392	Run B N = 392	Run C N = 392	Eluate 1 vs. Eluate 2 (Run A vs. Run B)		100 beads vs. 50 beads (Run B vs. Run C)
pgp3	42.6%	42.3%	42.3%	0.995	(0.985 − 1.000)	0.990	(0.975–1.000)
CT694	36.5%	37.0%	37.0%	0.967	(0.941–0.993)	0.989	(0.974–1.000)
Cp17	92.3%	91.8%	92.1%	0.947	(0.887–1.000)	0.983	(0.949–1.000)
Cp23	81.6%	81.9%	81.6%	0.915	(0.863–0.967)	0.957	(0.920–0.994)
LecA	71.7%	71.7%	72.2%	0.899	(0.850–0.947)	0.975	(0.950–0.999)
VSP3	57.4%	57.7%	56.9%	0.917	(0.877–0.957)	0.943	(0.909–0.976)
VSP5	45.4%	45.2%	42.6%	0.871	(0.822–0.920)	0.928	(0.890–0.965)

CI = confidence interval. Cohen’s kappa shown for pairwise comparisons of 392 children. Eluate 1 was taken from the first blood spot extension and eluate 2 from the second blood spot extension. The bead comparison (e.g., 50 vs. 100) refers to the number of bead reads per well used by the software algorithm.