Table 1. Characteristics and properties of antimicrobial peptides used in this study.
AMP codes | Sources | Molecular weights (Da) | Amino acid sequences | Net charges | Hydrophobicity (%) | pI | MIC values (μg/ml)e |
---|---|---|---|---|---|---|---|
S5 | Buthus martensii Kasch a | 1,448.79 | FIGAIARLLSKIF | 2 | 56.23 | 11.6 | >400 |
S52 | Buthus martensii Kasch a, # | 1,188.46 | FIGAIARLLSK | 2 | 66.67 | 11.6 | >400 |
S6 | Bovine myeloid cells b | 2,059.46 | GGLRSLGRKILRAWKKYG | 6 | 33.33 | 11.91 | >400 |
S61 | Bovine myeloid cells b,# | 2,002.41 | GGLRSLGRKILRAWKKY | 6 | 35.29 | 11.91 | 200 |
S62 | Bovine myeloid cells b,# | 1,839.24 | GGLRSLGRKILRAWKK | 6 | 37.5 | 12.44 | 200 |
S63 | Bovine myeloid cells b,# | 1,711.07 | GGLRSLGRKILRAWK | 5 | 40 | 12.43 | 200 |
KLK | Sarcophaga peregrina c | 1,322.81 | KLKLLLLLKLK | 4 | 63.64 | 11.15 | >400 |
KLK1 | Sarcophaga peregrina c,# | 1,194.66 | KLKLLLLLKL | 3 | 70 | 10.98 | 400 |
KLK2 | Sarcophaga peregrina c,# | 1,081.50 | KLKLLLLLK | 3 | 66.67 | 10.98 | >400 |
Pug-1 | Punica granatum d | 1,553.84 | LLKLFFPFLETGE | -1 | 61.54 | 4.15 | >400 |
Pug-2 | Punica granatum d | 587.67 | GAVGSVV | 0 | 57.14 | 3.65 | >400 |
Pug-3 | Punica granatum d | 452.5 | LGTY | 0 | 25 | 3.61 | >400 |
Pug-4 | Punica granatum d | 922.08 | FPSFLVGR | 1 | 62.5 | 10.59 | >400 |
Note: AMP, antimicrobial peptide; Da, daltons; pI, isoelectric points; MIC, minimal inhibitory concentration.
a Buthus martensii Kasch (scorpion venom) [44].
b bovine myeloid cells [45].
c Sarcophaga peregrina (flesh fly) [46].
d Punica granatum (Pomegranate peel) [47].
e In vitro screening antimicrobial activities of 13 AMPs against M. abscessus ATCC19977 strain. MIC values were measured in duplicate in two independent experiments.
# Novel modified AMPs by truncation of amino acid residues from its parent AMP from the current study.