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. 2021 Nov 15;16(11):e0255706. doi: 10.1371/journal.pone.0255706

Fig 5. The Kdm6a-null stroma promotes the expression of Kdm6a-null hematopoietic stem and progenitor cells.

Fig 5

(A and B) Experimental scheme of a non-competitive BM repopulation assay. (A) Whole BM cells from indicated donor mice (CD45.2) were transplanted into lethally-irradiated indicated recipient mice (CD45.2). (B) Recipient mice were given tamoxifen after 4 weeks of engraftment to overcome engraftment defect. The Tamoxifen dosing protocol used was 3 mg/day/mouse, MWF x 3 weeks. 6 weeks after tamoxifen treatment, BM cells were examined by flow cytometry and the percentage of Kdm6a floxed alleles was measured by q-PCR. Table shows the expected genotype for the BM and stroma cells of the corresponding cohort. (C) Quantification of the indicated stem cell compartments showed a significant increase in LSK and SLAM cell populations in cohort 1 as compared to all other cohorts. (D) Quantification of the indicated progenitor compartments showed an increase in GMPs and a decrease in MEPs in cohort 1 as compared to all other cohorts. Error bars represented mean ± s.d. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 by unpaired two-tailed Student’s t-test.