Table 3. Independent variables and their ranges used for zymogram optimization in the Box Behnken design.
Coded and actual levels U/g | ||||
---|---|---|---|---|
Independent variable | Factor | -1 | 0 | 1 |
Glucoamylase | x 1 | 269.65 | 284.83 | 300.00 |
Pullulanase | x 2 | 532.56 | 541.28 | 550.00 |
Maltase | x 3 | 2.00 | 2.60 | 3.20 |
Thermostable α-amylase | x 4 | 1,385.71 | 1,442.86 | 1,500.00 |
Medium temperature α-amylase | x 5 | 269.50 | 277.25 | 285.00 |
Cold-active α-amylase | x 6 | 1,965.00 | 1,982.50 | 2,000.00 |