Skip to main content
. 2021 Nov 15;16(11):e0259865. doi: 10.1371/journal.pone.0259865

Table 3. Independent variables and their ranges used for zymogram optimization in the Box Behnken design.

Coded and actual levels U/g
Independent variable Factor -1 0 1
Glucoamylase x 1 269.65 284.83 300.00
Pullulanase x 2 532.56 541.28 550.00
Maltase x 3 2.00 2.60 3.20
Thermostable α-amylase x 4 1,385.71 1,442.86 1,500.00
Medium temperature α-amylase x 5 269.50 277.25 285.00
Cold-active α-amylase x 6 1,965.00 1,982.50 2,000.00