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. 2021 Nov 15;16(11):e0259865. doi: 10.1371/journal.pone.0259865

Table 4. Effects of supplementation of exogenous carbohydrase on the reducing sugar release rate and the improved dry matter digestibility of diets for broilers aged 1 to 3 wk.

Item Content Quadratic regression equation P value Optimal supplement level, U/g Maximun RS, mg/g or improved DMD, %
Glucoamylase Supplement level, U/g 10.00 20.00 40.00 80.00 160.00 320.00 y = -0.001x2 + 0.539x + 2.786 <0.01 269.65 75.50
RSA, mg/g 6.87 15.71 22.47 37.91 65.91 77.85
Pullulanase Supplement level, U/g 10.00 20.00 40.00 80.00 160.00 320.00 y = -0.000001x2 + 0.106x - 0.998 <0.01 532.50 27.36
RSA, mg/g 0.12 1.22 3.16 6.29 13.70 22.27
Maltase Supplement level, U/g 0.16 0.31 0.63 1.25 2.50 5.00 y = -2.092x2 + 8.382x + 1.187 <0.01 2.00 9.58
RSA, mg/g 1.90 3.50 6.21 8.88 8.53 -9.10
Thermostable α-amylase Supplement level, U/g 80.00 160.00 320.00 640.00 1,280.00 2,560.00 y = -0.000007x2 + 0.019x - 0.945 <0.01 1,385.71 12.50
RSA, mg/g 0.99 1.95 4.16 8.57 13.30 5.10
Medium temperature α-amylase Supplement level, U/g 10.63 21.25 42.50 85.00 170.00 340.00 y = -0.0002x2 + 0.108x + 1.431 <0.01 269.50 15.96
RSA, mg/g 2.71 3.93 5.80 8.33 15.91 19.69
Cold-active α-amylase Supplement level, U/g 64.00 128.00 256.00 512.00 1,024.00 2,048.00 y = -0.00001x2 + 0.039x + 3.597 <0.01 1,965.00 42.21
RSA, mg/g 5.15 7.96 14.68 20.08 29.33 28.18
Glucoamylase Supplement level, U/g 12.50 25.00 50.00 100.00 200.00 400.00 y = -0.000004x2 + 0.002x + 0.217 <0.01 300.00 0.58
Improved DMD, % 0.23 0.28 0.32 0.43 0.53 0.57
Pullulanase Supplement level, U/g 25.00 50.00 100.00 200.00 400.00 800.00 y = -0.000003x2 + 0.003x + 0.408 <0.01 550.00 1.32
Improved DMD, % 0.51 0.59 0.67 0.91 1.29 1.16
Maltase Supplement level, U/g 0.16 0.31 0.63 1.25 2.50 5.00 y = -0.024x2 + 0.152x + 0.213 <0.01 3.20 0.46
Improved DMD, % 0.23 0.27 0.30 0.35 0.45 0.38
Thermostable α-amylase Supplement level, U/g 68.75 137.50 275.00 550.00 1,100.00 2,200.00 y = -0.0000002x2 + 0.0006x + 0.16 <0.01 1,500.00 0.61
Improved DMD, % 0.19 0.23 0.32 0.43 0.54 0.40
Medium temperature α-amylase Supplement level, U/g 10.00 20.00 40.00 80.00 160.00 320.00 y = -0.00002x2 + 0.011x + 0.131 <0.01 285.00 1.76
Improved DMD, % 0.28 0.32 0.48 1.03 1.39 1.71
Cold-active α-amylase Supplement level, U/g 68.75 137.50 275.00 550.00 1,100.00 2,200.00 y = -0.0000001x2 + 0.0004x + 0.128 <0.01 2,000.00 0.53
Improved DMD, % 0.15 0.18 0.27 0.35 0.50 0.64

RSA: Reducing sugar release rate; DMD: Dry matter digestibility.