Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Strain, strain background (Escherichia coli)	K1(RS218)	PMID:26205862		Provided by the Nizet lab
Strain, strain background (Acinetobacter baumannii)	AB 5075	PMID:24865555		Provided by the Nizet lab
Strain, strain background (Pseudomonas aeruginosa)	PAO1	PMID:10984043		This study
Strain, strain background (Staphylo coccus aureus)	Newman, USA300,113, 6538, 33,591	ATCC, PMID:17951380		This study
Strain, strain background (Staphylo coccus aureus)	NIAMS	PMID:28228596	Lesional skin of atopic dermatitis patients
Strain, strain background (Enterococcus faecium)	VRE 5998			Provided by Nizet lab
Strain, strain background (Klebsiella pneumoniae)	K1100	PMID:31353294		Provided by Nizet lab
Strain, strain background (Bacillus subtilis)	PY79	PMID:24356846		This study
Cell line (Homo-sapiens)	(NHEK) normal human epidermal keratinocytes	Thermo Fisher	C0015C
Antibody	Anti-Phospho-TBK1/NAK (Ser172) (Rabbit mAb)	Cell Signaling	Cat# D52C2	WB (1:1,000)
Antibody	Anti-TBK1/NAK (Rabbit mAb)	Cell Signaling	Cat# D1B4	WB (1:1,000)
Antibody	Anti-Phospho-IRF-3 (Ser396) (Rabbit mAb)	Cell Signaling	Cat# 4D4G	WB (1:1,000)
Antibody	Anti-IRF-3 (Rabbit mAb)	Cell Signaling	Cat# D83B9	WB (1:1,000)
Antibody	Anti-COX IV (Rabbit mAb)	Cell Signaling	Cat# 3E11	WB (1:1,000)
Antibody	LICOR IRDye 680RD Donkey anti-Goat IgG	Licor	Cat# LIC-926–68074	WB (1:10,000)
Antibody	LICOR IRDye 800CW Donkey anti-Rabbit IgG	Licor	Cat# LIC-925–32213	WB (1:10,000)
Sequence- based reagent	DEFB4A	Integrated DNA Technologies	PrimeTime predesigned qPCR primers	Assay ID# Hs.PT.58.40718840
Sequence- based reagent	GAPDH	Integrated DNA Technologies	PrimeTime predesigned qPCR primers	Assay ID# Hs.PT.39a.22214836
Sequence- based reagent	IL-6	Integrated DNA Technologies	PrimeTime predesigned qPCR primers	Assay ID# Hs.PT.58.40226675
Sequence- based reagent	CXCL10	Integrated DNA Technologies	PrimeTime predesigned qPCR primers	Assay ID# Hs.PT.58.3790956.g
Commercial assay or kit	Thermo Pierce Silver stain kit	Thermo Fisher	Cat. #: 24,612
Commercial assay or kit	iTaq universal SYBR green supermix	Biorad	Cat. #: 1725121
Commercial assay or kit	Pierce BCA Protein Assay Kit	Thermo Fisher	Cat. #: 23,225
Commercial assay or kit	Pierce LDH Cytotoxicity Assay Kit	Thermo Scientific	Cat. #: PI88954
Commercial assay or kit	Human IP-10 ELISA Set	BD Biosciences	Cat. #: 550,926
Commercial assay or kit	PureLink RNA Mini Kit	Thermo Fisher	Cat. #: 12183025
Commercial assay or kit	LIVE/DEAD BacLight Bacterial Viability Kit	Thermo Fisher	Cat. #: L7012
Commercial assay or kit	2 ml 96-well sterile deepWell plates	Fisher Scientific	Cat. #: 12-565-605
Chemical compound, drug	n-butanol 99.9%	Sigma Aldrich	Cat. #: 537,993
Chemical compound, drug	Crystal violet solution (1%)	Sigma Aldrich	Cat. #: V5265
Chemical compound, drug	Micrococcin P1	Cayman Chemical	Cat. #: 17,093
Chemical compound, drug	Poly I:C	InvivoGen	Cat. #: tlrl-pic
Chemical compound, drug	MALP-2	Enzo Life Sciences	Cat. #: ALX-162–027 C050
Chemical compound, drug	Precision Plus Protein Dual Xtra Prestained Protein Standard	Biorad	Cat. #: 1610377
Chemical compound, drug	Human keratinocyte growth supplement	Thermo Fisher	Cat. #: S0015
Chemical compound, drug	Trypsin/EDTA solution (TE)	Thermo Fisher	Cat. #: R001100
Chemical compound, drug	Defined trypsin inhibitor	Thermo Fisher	Cat. #: R007100
Chemical compound, drug	EpiLife medium with 60 μM calcium	Thermo Fisher	Cat. #: MEPI500CA
Chemical compound, drug	Antibiotic Antimycotic Solution (100 X)	Millipore Sigma	Cat. #: A5955
Chemical compound, drug	RIPA Lysis and Extraction Buffer	Fisher Scientific	Cat. #: PI89900
Chemical compound, drug	Halt Protease and Phosphatase Inhibitor Cocktail (100 X)	Thermo Fisher	Cat. #: 78,440
Chemical compound, drug	Intercept (PBS) Blocking Buffer	Licor	Cat. #: 927–70001
Software, algorithm	GraphPad Prism 7.03	GraphPad Software Inc
Software, algorithm	FlowJo V10	BD Biosciences