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. 2021 Nov 15;131(22):e152911. doi: 10.1172/JCI152911

Figure 5. ASAP regulates ATP synthase activity and enhances the interaction between ATP5A and ATP5C.

Figure 5

(A and B) The interaction between ATP5A and ATP5C was detected using Co-IP assays in HCT116 and RKO cells with overexpressed ASAP (A) or ASAP KO (B). (C) In vitro pulldown assay was performed using bacterial purified MBP-ATP5A-His and GST-ATP5C in the presence or absence of ASAP-His to confirm that ASAP directly enhances the interaction between ATP5A and ATP5C. (D and E) The activities of respiratory complex V (ATP synthase) were investigated in HCT116 and RKO cells with ASAP overexpressed (D) or ASAP KO (E). Data are presented as mean value ± SD. n = 6 biologically independent experiments. Independent samples; 2-sided Student’s t test. ***P < 0.001. (F) Schematic diagram showing the interaction between ASAP and ATP synthase. The ATP synthase structural model was constructed based on Yoshida et al. (67). Data are representative of 3 independent experiments (A–C).