Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens [human]) | TET1 | GenBank | RC218608 | cDNA were purchased from Origene Corp. |
| Gene (Homo sapiens [human]) | SIRT1 | GenBank | NM_012238 | |
| Gene (Homo sapiens [human]) | SIN3A | GenBank | NM_001145357 | |
| Cell line (Homo sapiens [human]) | HepG2 cell line | ATCC | HB-8065 | |
| Cell line (Homo sapiens [human]) | LO2 cell line | Cellosaurus | CVCL_6926 | |
| Cell line (Homo sapiens [human]) | HEK293T cell line | ATCC | CRL-11268 | |
| Transfected construct (human) | TET1 shRNA #1; shRNA #2 | lentivirus vector pLKO_SHC201 | Cat# SHC201 | Sequence: shRNA#1: CCCAGAAGATTT AGAATTGAT shRNA#2: CCTCCAGTCTT AATAAGGTTA |
| Transfected construct (human) | siRNA to TET1(SMARTpool) | Dharmacon/Thermo Fisher Scientific | Cat# D-014635 | |
| Transfected construct (human) | siRNA: non-target in control (GL2 Duplex) | Dharmacon/Thermo Fisher Scientific | 1022070 | |
| Sequence-based reagent | G6PC. S | This paper | RT-PCR primers | GCAGGTGTATAC TACGTGATGGT |
| Sequence-based reagent | G6PC. A | This paper | RT-PCR primers | GACATTCAAGC ACCGAAATCTG |
| Sequence-based reagent | PPARGC1A. S | This paper | RT-PCR primers | TGACTGGCGTC ATTCAGGAG |
| Sequence-based reagent | PPARGC1A. A | This paper | RT-PCR primers | CCAGAGCAG CACACTCGAT |
| Sequence-based reagent | SLC2A4. S | This paper | RT-PCR primers | GGGAAGGAAA AGGGCTATGCTG |
| Sequence-based reagent | SLC2A4. A | This paper | RT-PCR primers | CAATGAGGAAC CGTCCAAGAATG |
| Sequence-based reagent | TET1. S | This paper | RT-PCR primers | GGAATGGAAG CCAAGATCAA |
| Sequence-based reagent | TET1. A | This paper | RT-PCR primers | ACTCCCTAAG GTTGGCAGTG |
| Sequence-based reagent | PCK1.S | This paper | RT-PCR primers | ATCCCCAAAAC AGGCCTCAG |
| Sequence-based reagent | PCK1. A | This paper | RT-PCR primers | ACGTACATGGT GCGACCTTT |
| Sequence-based reagent | ACTB. S | This paper | RT-PCR primers | TACTGCCCTG GCTCCTAGCA |
| Sequence-based reagent | ACTB. A | This paper | RT-PCR primers | GCCAGGATAGA GCCACCAATC |
| Antibody | Anti-SIRT1 (Rabbit polyclonal) | Millipore | Cat# 05–1243 | WB (1:2000) |
| Antibody | Anti-TET1 (Rabbit polyclonal) | Millipore | Cat# 09–872 | WB (1:1000) |
| Antibody | Anti-SIN3A (Rabbit polyclonal) | Abcam | Cat# ab3479 | WB (1:2000) |
| Antibody | Anti-AMPK (Rabbit polyclonal) | Abcam | Cat# ab3759 | WB (1:2000) |
| Antibody | Anti-AMPK T172 (Rabbit polyclonal) | Cell Signaling | Cat# 2535 | WB (1:2000) |
| Antibody | Anti-p53 (Rabbit polyclonal) | Abcam | Cat# ab26 | WB (1:2000) |
| Antibody | Anti-p53 acetyl 382 (Rabbit polyclonal) | Abcam | Cat# ab75754 | WB (1:2000) |
| Antibody | Anti-FOXO1 (Rabbit polyclonal) | Abcam | Cat# ab52857 | WB (1:2000)IF (1:500) |
| Antibody | Anti-PGC-1α (Rabbit polyclonal) | Abcam | Cat# ab54481 | WB (1:2000)IF (1:500) |
| Antibody | Anti-Histone H3 (Mouse monoclonal) | Abcam | Cat# ab1791 | WB (1:4000) |
| Antibody | Anti-lysine acetylated antibody (Rabbit polyclonal) | Cell Signaling | Cat# 9441 | WB (1:2000) |
| Antibody | Anti-LaminB (Rabbit polyclonal) | Santa Cruz | Cat# sc-6216 | WB (1:4000) |
| Antibody | Anti-GAPDH (Mouse monoclonal) | Santa Cruz | Cat# sc-32233 | WB (1:4000) |
| Antibody | Anti-5hMC (Rabbit polyclonal) | Active Motif | Cat# AB_2630381 | IP 4 µg/1 mg total lysate |
| Chemical compound, drug | Metformin | Sigma Aldrich | Cat# PHR108 | |
| Chemical compound, drug | AICAR | Sigma Aldrich | Cat# A9978 | |
| Chemical compound, drug | RSV | Sigma Aldrich | Cat# R5010 | |
| Chemical compound, drug | EX527 | Sigma Aldrich | Cat# E7034 | |
| Chemical compound, drug | SRT2104 | SellectChem | Cat# S7792 | |
| Commercial assay or kit | Pyruvate Assay Kit | Sigma Aldrich | Cat# MAK071 | |
| Commercial assay or kit | Glucose (Go) Assay Kit | Sigma Aldrich | Cat# GAGO20 | |
| Commercial assay or kit | SIRT1 Activity Assay Kit | Abcam | Cat# ab156065 | |
| Software, algorithm | SPSS; Image J | SPSS; Image J | RRID:SCR_002865; RRID:SCR_003070 | |
| Strain, strain background (Escherichia coli) | BL21(DE3) | Sigma Aldrich | CMC0016 | |
| Other | DAPI stain | Invitrogen | D1306 | (1 µg/ml) |
| Other | Lipo3000 | Invitrogen | Cat# L3000001 | |
| Other | Tet1 Knockout mice | Jackson | Cat# 017358 |