| Strain, strain background (M. brevicollis) |
M. brevicollis
|
ATCC PRA-258 |
PMID:18276888
|
|
| Genetic reagent, (M. brevicollis) |
M. brevicollis STING
–
|
This study |
|
STING–knockout strain; cell line maintained by A. Woznica |
| Transfected construct (M. brevicollis) |
pEFL5’-pac-P2A-STING-mTFP-3'act |
This study |
|
Construct to express M, brevicollis STING fused to mTFP; can be obtained from A. Woznica |
| Transfected construct (M. brevicollis) |
pEFL5’-pac-P2A-mCherry-Atg8-–3'act |
This study |
|
Construct to express mCherry fused to M. brevicollis Atg8; can be obtained from A. Woznica |
| Strain, strain background (Flavobacterium) |
Flavobacterium sp. |
This study |
|
Isolated from MX1 (ATCC PRA-258) culture; can be obtained from A. Woznica |
| Strain, strain background (Pseudomonas aeruginosa) |
PAO1 |
ATCC 15692 |
PMID:13961373
|
|
| Strain, strain background (P. aeruginosa, transgenic strain) |
PAO1-GFP |
ATCC 15692GFP |
PMID:9361441
|
|
| Antibody |
anti-choano STING (rabbit polyclonal) |
This study |
|
Generated by Pacific Immunology; dilution (1:200) for IF, (1:2000) dilution for WB; can be obtained from A. Woznica |
| Antibody |
Anti-mCherry 16D7 (rat monoclonal) |
Invitrogen |
Cat# M11217
|
(1:2000) dilution for WB |
| Antibody |
Anti-human Tubulin E7 (Mouse monoclonal) |
Developmental Studies Hybridoma Bank |
Cat# AB_2315513 |
(1:200) dilution for IF |
| Antibody |
Alpha-human tubulin (Mouse monoclonal) |
Sigma Aldrich |
Cat # T64074
|
(1:7000) dilution for WB |
| Chemical compound, drug |
2’3’ cGAMP |
Cayman Chemical |
Cat# 19,887 |
|
| Chemical compound, drug |
3’3’ cGAMP |
Cayman chemical |
Cat# 17,966 |
|
| Sequence-based reagent |
STING556 gRNA |
This study |
Guide RNA |
TTTCGGGATTCAGATGTGGG |
| Sequence-based reagent |
STING locus PCR primers |
This study |
PCR primers |
F: 5’ ATG ATG GTT AAT CTC TCT GAT CTT TCA CAT C 3’ R: 5’ TTA TGG CAT CGC ATA CTG GTC C 3’ |
| Commercial assay or kit |
SG Cell Line 4D-NucleofectorTM X Kit S |
Lonza, |
Cat# V4XC-3032 |
|
