Figure 7. Sympathetic-sensory signalling and influence of ageing.

(a–b) RT-PCR CT analysis shows the expression and fold change of Trpa1 and Trpm8 in young and aged SG normalised to three housekeeping genes collected from the cervical and thoracic paravertebral region. (c) The western blot analysis of TRPM8 in SG of young and aged mice. All results are shown as mean ± s.e.m. (n = 5–7) *p < 0.05, **p < 0.01. (Two-tailed Student’s t-test). (d) Shows the raw trace of intracellular calcium change (mean ± s.e.m.) induced by menthol (300 µM) shown by the ratiometric fura-2 fluorescence change over time. (e) Maximum % change in fluorescence change from baseline caused by menthol in the presence and absence of AMTB (10 µM). (f) Shows the raw trace of intracellular calcium change (mean ± s.e.m.) induced by AITC (200 µM) shown by the ratiometric fura2 fluorescence change over time. (g) Maximum % change in fluorescence change from baseline caused by AITC in the presence and absence of A967079 (10 µM). Arrow represents the time-point of agonist injection. n = 5 independent experiments, where each treatment was a mean of replicate values. All results are shown as mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.01. (One-way ANOVA with Tukey’s post hoc test or Two-tailed Student’s t-test).

Figure 7—figure supplement 1. Sympathetic-sensory signalling in young and aged mice.

(a–c) RT-PCR CT analysis shows fold change of α_2a adrenoceptor, α_2c adrenoceptor and Trpv1 in young and aged mice normalised to three housekeeping genes in (DRG). Results are shown as mean ± s.e.m. (n = 14–16) *p < 0.05, ***p < 0.001. (Two-tailed Student’s t-test).

Figure 7—figure supplement 2. Characterisation of sympathetic markers.

(a) RT-PCR CT analysis shows fold change of the sympathetic nerve marker Dopamine β-hydroxylase (Dbh) in DRG and SG normalised to three housekeeping genes. (b) The western blot analysis of the sympathetic nerve marker tyrosine hydroxylase (TH) in SG, DRG and kidney. Results are shown as mean ± s.e.m. (n = 3).