(
A) AlphaFold model of full-length SMG8 (UniProt:
Q8ND04) colored by per-residue confidence score (pLDDT) (
Jumper et al., 2021). The previously unmodeled SMG8 C-terminus is indicated. (
B) AlphaFold model of the SMG8 C-terminus (dark blue) rigid-body fitted into the isolated cryo-EM density and fused to the modeled N-terminal part of SMG8 (light blue), shown in two related views. Regions with a pLDDT<50 were deleted from the model. (
C) Intra cross-links of the SMG8 C-terminus mapped on the model, as described in
Figure 4—figure supplement 1. A table shows the corresponding measured distances. (
D) New model of the SMG1-8-9 complex, now including the SMG8 C-terminal region, shown from two different perspectives (PDB identifier: 7PW5). cryo-EM, cryo-electron microscopy.