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. 2021 Oct 26;10:e72353. doi: 10.7554/eLife.72353

Figure 5. Hypothetical model of SMG1 kinase regulation.

Figure 5.

Structural and biochemical data suggest different layers of regulation on SMG1 kinase activity. Upon ATP binding (orange), the SMG1 kinase adopts an autoinhibited conformation (step 1)—mediated by the concerted action of the SMG1 insertion domain in cis and the SMG8 C-terminus in trans (dotted arrow). The presence of the correct substrate and likely other factors/cues (indicated by X) then trigger the release of the autoinhibition and activity of the kinase toward UPF1 (step 2).