| Gene (Homo sapiens) |
SMG1 |
Shigeo Ohno lab |
Uniprot Q96Q15
|
|
| Gene (H. sapiens) |
SMG8 |
Shigeo Ohno lab |
Uniprot Q8ND04
|
|
| Gene (H. sapiens) |
SMG9 |
Shigeo Ohno lab |
Uniprot Q9H0W8
|
|
| Cell line (H. sapiens) |
HEK293T |
ATCC |
|
|
| Strain, strain background (Escherichia coli) |
BL21 Star (DE3) pRARE |
EMBL Heidelberg Core Facility |
|
Electrocompetent cells |
| Peptide, recombinant protein |
UPF1-LSQ (peptide 1078) and derivative |
In-house as described in doi: https://elifesciences.org/articles/57127
|
|
|
| Chemical compound, drug |
SMG1 inhibitor |
Robert Bridges, Rosalind Franklin University of Medicine and Science, and the Cystic Fibrosis Foundation |
|
|
| Chemical compound, drug |
AMPPNP |
Sigma-Aldrich |
|
|
| Chemical compound, drug |
ATP |
Sigma-Aldrich |
|
|
| Software, algorithm |
SerialEM |
https://bio3d.colorado.edu/SerialEM/
|
|
|
| Software, algorithm |
Focus |
https://focus.c-cina.unibas.ch/
wiki/doku.php
|
v1.1.0 |
|
| Software, algorithm |
RELION |
doi: 10.7554/eLife.42166
|
RELION 3.0 |
|
| Software, algorithm |
Cryosparc |
doi: 10.1038/nmeth.4169
|
Cryosparc2 |
|
| Software, algorithm |
UCSF Chimera |
UCSF, https://www.cgl.ucsf.edu/chimera/
|
|
|
| Software, algorithm |
UCSF ChimeraX |
UCSF, https://www.rbvi.ucsf.edu/chimerax/
|
|
|
| Software, algorithm |
COOT |
http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
|
|
|
| Software, algorithm |
PHENIX |
https://www.phenix-online.org/
|
PHENIX 1.17 |
|
| Software, algorithm |
Molprobity |
Duke Biochemistry, http://molprobity.biochem.duke.edu/
|
|
|
| Software, algorithm |
PyMOL |
PyMOL Molecular Graphics System, Schrodinger LLC |
PyMOL 2.3.2 |
|
