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. 2021 Nov 8;2021:7031492. doi: 10.1155/2021/7031492

Figure 1.

Figure 1

Plasmid construction: third-generation lentiviral vector was used. In this construct, expression of the BMP2-mir424 and copGFP-Puromycin was controlled under pCMV and eEF1 promoters, respectively. The copGFP gene was linked with the T2A peptide to Puromycin-resistant gene. The transfer vector generated two transcripts, and then, a miR and three proteins, BMP2, copGFP, and the puromycin-resistant marker, were made. The copGFP and the puromycin-resistant markers were used for monitoring and selection in transfection and transduction steps.