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. 2021 Nov 8;2021:7031492. doi: 10.1155/2021/7031492

Figure 3.

Figure 3

Quantitative polymerase chain reaction and ELISA analysis of Wharton's jelly stem cells. (a) miR424 gene expression was increased in miR424, BMP2, and BMP2-miR424 870.41, 300.16, and 600.36 folds, respectively. (b) Moreover, BMP2 gene expression elevated in all studied groups (miR424: 423.25, BMP2: 1015.35, and BMP2-miR424: 1700.11). (c) According to the results of ELISA analysis, the amount of Osteocalcin in the culture medium was alternately increased in studied groups. As shown in this figure, these amounts were increased in miR424, BMP2, and BMP2-miR424 groups. This increase was the highest in the BMP2-miR424 group. (p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001).