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. 2021 Nov 8;2021:1805147. doi: 10.1155/2021/1805147

Figure 1.

Figure 1

Baicalin ameliorates pyroptosis in Mtb-infected macrophages. (a) Levels of GSDMD-N protein were determined by western blotting in RAW264.7 cells. (b) Levels of GSDMD-N proteins were analyzed by western blot measurement in primary peritoneal macrophage cells. (c, d) Cell death was evaluated applying the Lactate Dehydrogenase (LDH) Cytotoxicity Assay Kit in RAW264.7 cells and in primary peritoneal macrophage cells. (e) Transfection efficiency of siRNAs was measured by western blotting analysis in RAW264.7 cells. (f, g) Cell death after GSDMD siRNA was evaluated applying the Lactate Dehydrogenase (LDH) Cytotoxicity Assay Kit in RAW264.7 cells and in primary peritoneal macrophage cells. Data are shown as mean ± SD of three independent experiments. ∗p < 0.05; ∗∗∗p < 0.001; NS: nonsignificant.