KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| E. coli BL21(DE3) | Novagen | Cat#70235 |
| Chemicals, peptides, and recombinant proteins | ||
| (2S,4R)-1-((S)-2-amino-3,3-dimethylbutanoyl)-4-hydroxy-N-(4-(4-methylthiazol-5-yl)benzyl)pyrrolidine-2-carboxamide | Millipore Sigma | Cat#901490 CAS: 1448189–80-7 |
| 1-(9H-fluoren-9-yl)-3-oxo-2,7,10,13-tetraoxa-4-azapentadecan-15-oic acid | Millipore Sigma | Cat#CDS020947 CAS: 139338–72-0 |
| (1-[bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxide hexafluorophosphate (HATU) | Millipore Sigma | Cat#445460 CAS: 148893–10-1 |
| (S)-2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetic acid | Millipore Sigma | Cat#SML1524 CAS: 1268524–70-4 |
| MZ1 | This paper (as Zengerle et al., 2015) | N/A |
| Cellgro Miller’s LB broth | Corning | Cat#46–050-CM |
| Cellgro Terrific Broth® (TB) | Corning | Cat#46–055-CM |
| Chloramphenicol | Millipore Sigma | Cat#C0378 |
| Kanamycin Sulfate | Millipore Sigma | Cat#60615 |
| Isopropyl β-d-thiogalactopyranoside (IPTG) | Millipore Sigma (Roche) | Cat#10724815001 |
| cOmplete™ EDTA-free protease inhibitor cocktail | Millipore Sigma (Roche) | Cat#11873580001 |
| Benzonase® endonuclease | Millipore Sigma (Merck KGaA) |
Cat#E1014 |
| AcTEV protease | ThermoFisher | Cat#12575015 |
| His6-TVMV protease | Nallamsetty, et al., 2004 | N/A |
| Ammonium acetate, 99.999% purity | Millipore Sigma | Cat#372331; CAS: 631–61-8 |
| Ethylene diamine diacetate | Millipore Sigma | Cat#420352; CAS: 38734–69-9 |
| hBRD4 (333–460) | This paper (as Gadd et al., 2017) | Uniprot Q60885 |
| VHL (54–213) | This paper (as Gadd et al., 2017) | Uniprot P40337 |
| Elongin C (17–112) | This paper (as Gadd et al., 2017) | Uniprot Q15369 |
| Elongin B (1–104) | This paper (as Gadd et al., 2017) | Uniprot Q15370 |
| Deposited data | ||
| hBRD4 | Gadd et al.,2017 | PDB: 2OUO |
| VCB | Gadd et al.,2017 | PDB: 1VCB |
| hBRD4-MZ1-VCB complex | Gadd et al.,2017 | PDB: 5T35 |
| Recombinant DNA | ||
| pET28-His6-VHL54–213 | Galdeano et al., 2014 | N/A |
| pACYC-Duet-1-ElonginC17–112-ElonginB1–104 | Galdeano et al., 2014 | N/A |
| pET28-His6-BRD4333–460 | Gadd et al., 2017 | N/A |
| Software and algorithms | ||
| MassLynx 4.2 | Waters Corp. | https://www.waters.com |
| CIUSuite 2.0 | Polasky et al., 2019 | https://sites.lsa.umich.edu/ruotolo/software/ |
| Intact Mass™ | Protein Metrics Inc. | https://proteinmetrics.com |
| Other | ||
| 12 T FT-ICR MS | Bruker Daltonics | https://www.bruker.com |
| Synapt G2 HDMS | Waters Corporation | https://www.waters.com |
| Exactive Plus EMR orbitrap MS | Thermo Scientific | https://www.thermofisher.com |