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. 2021 Nov 8;7(11):1461–1474. doi: 10.1038/s41477-021-01008-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, BSAS of the 35S:EVD transgene in independent biological replicates of T1 plants. The bar displays the methylation percentage and the error bars represent the 95% confidence interval calculated using the Wilson score interval method. n is the number of total cytosines assayed for each amplicon. H is A, C or T. b, RdDM strength plot of DNA methylation. RdDM strength was measured by calculating the length of stretches of consecutively methylated cytosines along individual sequence reads. All box plots in this paper represent 25th and 75th percentile values with whiskers at the 10th and 90th percentiles, the median is represented by a line and the mean is denoted by a filled circle.