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. 2021 Nov 8;7(11):1461–1474. doi: 10.1038/s41477-021-01008-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, Two-component system with siRNA production from a T1 inverted repeat (IR) transgene targeting the endogenous gene SQK1 for RdDM. b, Heatmap of small RNA sequencing. c, BSAS for T1 transgenic SQK1-IR plants. The data display is the same as Fig. 1a. d, Box plots of RdDM strength for plants from c. Box plot percentiles are the same as in Fig. 1b.