Table 1.
Error rates (per base) for thermophilic DNA polymerases
| Origin | Reaction | Temperature (°C) | Substitution rate (base−1) | Deletion rate (base−1) | Insertion rate (base−1) | Bases counted |
|---|---|---|---|---|---|---|
| Thermophile | Q5NB | 72 | 4.44 × 10−6 | 9.50 × 10−7 | 1.37 × 10−7 | 15,536,120 |
| Q5 | 30 | 6.04 × 10−6 | 7.45 × 10−7 | 5.31 × 10−7 | 6,001,934 | |
| Q5 | 72 | 5.38 × 10−6 | ND | ND | 6,340,445 | |
| Taq | 30 | 6.32 × 10−5 | 2.27 × 10−6 | 2.01 × 10−6 | 6,497,391 | |
| Taq | 50 | 2.18 × 10−4 | 2.18 × 10−5 | 3.53 × 10−6 | 6,320,921 | |
| PhusionNB | 30 | 2.51 × 10−5 | ND | 8.32 × 10−7 | 2,064,657 | |
| PhusionNB | 50 | 8.11 × 10−6 | ND | ND | 478,302 | |
| PhusionNB | 72 | 5.05 × 10−6 | ND | ND | 10,515,198 | |
| Phusion | 30 | 2.02 × 10−5 | 2.32 × 10−6 | 4.81 × 10−7 | 6,479,246 | |
| Phusion | 50 | 3.64 × 10−5 | 2.46 × 10−6 | 4.84 × 10−7 | 7,260,714 | |
| Phusion | 72 | 1.24 × 10−5 | ND | 5.11 × 10−7 | 6,182,116 |
NB denotes the reaction carried out in the native buffer supplied by the manufacturer.
ND denotes the error frequency below the detectable error baseline.