Table 3.
Error rates (per base) for mesophilic DNA polymerases
| Origin | Reaction | Temperature (°C) | Substitution rate (base−1) | Deletion rate (base−1) | Insertion rate (base−1) | Bases counted |
|---|---|---|---|---|---|---|
| Mesophile | KleExo- | 10 | 4.20 × 10−5 | 9.50 × 10−7 | ND | 6,370,293 |
| KleExo- | 20 | 6.91 × 10−5 | 7.45 × 10−7 | 5.31 × 10−7 | 2,000,239 | |
| KleExo- | 37 | 1.07 × 10−3 | ND | ND | 3,472,537 | |
| KleExo- | 50 | 1.38 × 10−3 | 2.27 × 10−6 | 2.01 × 10−6 | 3,408,561 | |
| KleLF | 10 | 8.53 × 10−6 | 2.18 × 10−5 | 3.53 × 10−6 | 5,822,486 | |
| KleLF | 20 | 1.56 × 10−5 | ND | 8.32 × 10−7 | 5,522,080 | |
| KleLF | 37 | 2.99 × 10−5 | ND | ND | 4,317,523 | |
| KleLF | 50 | 2.00 × 10−5 | ND | ND | 5,415,816 | |
| T4 | 10 | 3.25 × 10−5 | 2.32 × 10−6 | 4.81 × 10−7 | 6,168,596 | |
| T4 | 37 | 5.92 × 10−5 | 2.46 × 10−6 | 4.84 × 10−7 | 6,093,149 | |
| T4 | 50 | 1.45 × 10−5 | ND | 5.11 × 10−7 | 6,117,463 |
ND denotes the error frequency below the detectable error baseline.