Figure 4.

Labeling schemes for the discrimination between proliferating and quiescent subpopulations of daughter cells.A, double labeling in separate experimental groups (adapted from Takahashi et al. (16, 117)). B, triple labeling in the same experimental group (adapted from Podgorny et al. (41)). G₁, S, G₂, and M, phases of the cell cycle; P, proliferative fraction; Q, quiescent fraction; t, the time interval during which the second label is repeatedly delivered; t₃, the time point when the third label is delivered; T_{cell cycle}, cell cycle length; T_S-phase, S-phase duration; Δt₁₋₂, a time interval between the first and the second label pulses.