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. 2021 Nov 2;11:673780. doi: 10.3389/fonc.2021.673780

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Copyright © 2021 Wang, Su, Cheng and Ji

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SPAG5 knockdown decreased CDH2 expression in glioma cells. The expression of (A) CDH2 and (B) SPAG5 in cells transfected with SPAG5-shRNA were measured by western blotting. The HCS proliferation screening analysis of (C) U-87 cells and (D) U251 cells. ShRNA lentivirus-infected (E) U-87 and (F) U251 cells were cultured for 5 d and used in MTT assay. The absorption rate of light at wavelength of 490 nm was compared with time in each group. OD490 reflects the number of active cells. The invasion ability of transwell transferred (G) U-87 and (H) U251 cells in each experimental group was compared with that in the control group. NC+NC: Parental glioma cells+Vector; KD+NC: Parental glioma cells+Knockdown-SPAG5+Vector; KD+OE: Parental glioma cells+Knockdown-SPAG5+overexpression-CDH2. Results were expressed as mean ± SD from three independent experiments. vs NC+NC group, **P < 0.01; vs KD+NC group, ^&&P < 0.01.