Table 2.

Gene quantification (VC1376, thyA, and recA) by qPCR and ddPCR from a 10-fold serial dilution of genomic DNA of V. cholerae strain C6706.

qPCR
Concentration of Template	VC1376		thyA		recA
	Cq mean	SD	Cq mean	SD	Cq mean	SD
10 ng/μL	16.11	0.08	16.73	0.16	14.17	0.11
100 pg/μL	23.21	0.08	23.48	0.17	21.27	0.12
10 pg/μL	26.93	0.10	27.18	0.19	25.01	0.18
1 pg/μL	29.71	0.15	30.25	0.21	27.90	0.48
100 fg/μL	32.64	0.45	32.33	0.86	31.52	0.64
10 fg/μL	35.58	0.24	35.02	0.96	34.06	0.61
1 fg/μL	UD	UD	UD	UD	UD	UD
R2	0.997		0.995		0.999
ddPCR
Concentration of Template	VC1376		thyA		recA
	copies/μL*	SD	copies/μL*	SD	copies/μL*	SD
10 ng/μL	ULOD	ULOD	ULOD	ULOD	ULOD	ULOD
100 pg/μL	913.00	18.38	1253.00	16.97	711.50	19.09
10 pg/μL	118.00	7.07	119.50	9.19	64.30	12.30
1 pg/μL	10.70	0.71	11.45	0.92	8.65	0.49
100 fg/μL	1.20	0.14	1.60	0.42	1.45	0.21
10 fg/μL	0.17	0.03	0.22	0.02	0.27	0.02
1 fg/μL	0.03	0.02	0.07	0.01	0.08	0.02
R2	0.997		0.992		0.999

*The amount of DNA in each reaction.

UD, undetected. ULOD, DNA concentration at which the signal of the assay was saturated (>20,000 copies in the reaction mixture).