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. 2021 Nov 15;12:6565. doi: 10.1038/s41467-021-26851-2

Fig. 2. Colocalization of PQBP1 and incorporated Tau in microglia.

Fig. 2

a Immunocytochemistry of primary microglia prepared from C57BL/6 mice cultured with Tau 410, Tau 441, Tau 410 P179A, Tau 410 P216A, or Tau 410 P179A/P216A proteins in the medium. Colocalization of PQBP1 and extrinsic tau was detected frequently. The experiments were repeated four times independently with similar results. b In vitro polymerization of Tau 410, Tau 441, Tau 410 P179A, Tau 410 P216A, and Tau 410 P179A/P216A full-length proteins. Only Tau 441 and Tau 410 P216A partially formed fibrils after 10 days of incubation, while the other full-length protein did not. The experiments were repeated four times independently with similar results. c Images of normal microglia before and after addition of polymerized or non-polymerized Tau 410, Tau 441, Tau 410 P179A, Tau 410 P216A, or Tau 410 P179A/P216A, among which three species are shown in Supplementary Movies 1–3. h hour.