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. 2021 Nov 15;12:6565. doi: 10.1038/s41467-021-26851-2

Fig. 7. PQBP1-Tau interaction is essential for in vivo activation of microglia.

Fig. 7

a Protocol of injection of Tau 410 or Tau 410 PQBP1-binding mutant monomers (0.5 μg/μl, 4.0 μl/injection, 4.0 μg/mouse) or equivalent PBS to bilateral entorhinal cortices of non-induced or tamoxifen-induced Pqbp1-cKO mice. Five groups were used for analyses. b, c Immunohistochemistry of Iba1-positive microglia with PQBP1 + tau, cGAS+tau, NFκB-p65+tau colocalization in injected area of non-induced Pqbp1-cKO, and tamoxifen-induced Pqbp1-cKO. Quantitative analyses of PQBP1-Tau colocalized microglia, cGAS-tau colocalized microglia, and activated microglia (nuclear NFκB-positive) among Tau-incorporating microglia are shown in the graphs on the right. N = 10 (visual fields, from 3 mice). ##P < 0.01 in Tukey’s HSD test. d, e RT-qPCR of Tnf and Isg54 induction by Tau 410 and the PQBP1-binding mutants with entorhinal cortex tissues prepared from non-induced or tamoxifen-induced Pqbp1-cKO mice. N = 3. ##P < 0.01, n.s.P > 0.05 in Tukey’s HSD test (two-sided, multiple comparison). Box plots show the median, quartiles, and whiskers that represent data outside the 25th to 75th percentile range.