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. 2021 Nov 15;11:22238. doi: 10.1038/s41598-021-01731-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of immunoaffinity purified hTAS1R2. FLAG-tagged hTAS1R2 was solubilized in PBS containing 2% LMNG and captured using the EZview Red anti-FLAG M2 affinity gel. After elution with FLAG peptide, the eluate was collected, concentrated and subjected to SDS-PAGE followed by (A) staining with Coomassie blue and (B) by western blotting using mouse anti-FLAG M2 primary antibody and goat anti-mouse horseradish peroxidase conjugated secondary antibody. Full-length gels/blots are presented in Supplementary Fig. S3.