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. 2021 Nov 15;11:22215. doi: 10.1038/s41598-021-01671-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Changes induced by 5-azacytitidine (AC) tiller treatment in anther protein profiles (at least ± 2-fold change, p ≤ 0.05) of two doubled haploid (DH) lines of triticale, significantly differed in their responsiveness to microspore embryogenesis (responsive DH28 and recalcitrant DH19). AC was applied at concentrations of 2.5 µM (AC2.5), 5.0 µM (AC5.0) and 10 µM (AC10) during low temperature tillers treatment. Number of up-regulated (a) and down-regulated (b) proteins after AC treatment in comparison to the control (3 weeks at 4 °C); (c, d) Venn diagrams shows the overlap of the differentially expressed proteins following AC treatment. Quantitative Venn diagrams were constructed using a web application BioVenn accessible at http://www.biovenn.nl/index.php, but the original colours of the graphs were modified.