TABLE 1.

The imaging conditions of individual human oocyte samples.

				FIB settings			SEM settings				Dataset characteristics
Maturation stage	Sample ID	FIB-SEM microscope	Imaging time (h)	FIB type	Trench milling (nA)	Slicing (nA)	Detector type	Voltage (kV)	Beam current (pA)	Dwell time (μs/px)	Number of slices	Pixel XY (nm)	Pixel Z (nm)	XY pixel size	XY area (nm)	Z area (nm)
GV	MU-59	Amber X	39,8	Xe	300	100	LE-BSE	5	300	32	322	30	90	3,165 × 1,452	94,950 × 43,650	28,980
	MU-107	Solaris X	43,5	Xe	300	100	LE-BSE	4	300	32	222	40	100	4,224 × 1,674	168,960 × 66,960	22,200
	MU-158	Amber	49,4	Ga	50-85	20	LE-BSE	5	500	10	636	30	90	4,899 × 2,394	146,970 × 71,820	57,240
MI	MU-73	Amber	89,8	Ga	50-85	20	LE-BSE	5	600	10	1,294	30	90	4,124 × 2,228	123,720 × 66,840	116,460
	MU-75	Amber	76,2	Ga	50-85	20	LE-BSE	5	300	10	1,125	30	90	4,416 × 2,622	132,480 × 78,660	101,250
	MU-77	Solaris X	5,3	Xe	300	100	LE-BSE	4	300	32	120	40	100	1,364 × 1,462	54,560 × 58,480	12,000
MII	MU-89	Amber X	75,3	Xe	300	100	LE-BSE	3	400	30	237	30	90	5,043 × 3,315	151,290 × 99,450	21,330
	MU-108	Amber X	22,7	Xe	300	100	Axial	5	300	32	451	40	100	2,461 × 1,715	98,440 × 68,600	45,100
	MU-113	Amber X	55,1	Xe	300	100	Axial	3	300	19,2	494	40	40	3,100 × 2,152	124,000 × 86,080	19,760