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. 2021 Nov 15;12:6604. doi: 10.1038/s41467-021-26923-3

Fig. 5. eIF4A promotes PIC scanning in an ATPase-dependent manner.

Fig. 5

a Effects of Hippuristanol (Hippu) or Pateamine A (PatA) treatment on the translation efficiency of Fluc mRNA reporters with ultra-short 5ʹ UTR (top) or those containing three AUG codons near the 5ʹ end (bottom). Transfected MEF cells were treated with 200 nM Hippu or 50 nM PatA and Fluc activities were quantified by real-time luminometry. Error bars: mean ± SEM; n = 3 biological replicates. Two-tailed t-test, * p < 0.05; ** p < 0.01. b Effects of Hippu or PatA treatment on 5ʹ UTR ribosome occupancy revealed by Ribo-seq. Scatter plots show the correlation of read ratio (5ʹ UTR/CDS) between vehicle control and treatment (50 nM PatA: top; 200 nM Hippu: bottom) for 2 h (left) or 8 h (right). c Effects of Hippu or PatA treatment on CDS ribosome occupancy revealed by Ribo-seq. From the same data sets as (b), CDS ribosome occupancies on individual transcripts were normalized to the number of mitochondrial reads and plotted as cumulative fractions. d A representative gene (Rps5) shows differential 5ʹ UTR read density (red) relative to the CDS (dark gray) after treatment with eIF4A modulators. Source data are provided as a Source Data file.