FIGURE 8.

Enhancing SUMOylation does not change the fraction of Kv4.2g associated with α-adaptin. HEK cells were co-transfected with the TC components, and SUMOylation was (+) or was not (–) increased by co-expressing SUMO2 and Ubc9. Kv4.2g IP was obtained from cell lysates using an anti-GFP antibody, and IP products were resolved with PAGE. Western blots were probed for α-adaptin and then stripped and re-probed for Kv4.2g. (A) Representative western blots for α-adaptin and Kv4.2g for control and enhanced SUMOylation treatment groups. (B) Bar graphs plotting the mean ± SEM of α-adaptin that co-IPs with Kv4.2g for each treatment group [OD α-adaptin ÷ OD Kv4.2g]. Each data point is one independent experiment. TC, 0.02 ± 0.0035 vs. TC + SUMO2 + Ubc9, 0.02 ± 0.0033; t-test p = 0.657.