Fig. 6. Implementation of ApCB with aptamer TLS11a in an H22 tumor-bearing model.

a Preparation of ApCB by conjugating VNP with TLS11a via amide condensation. b Flow cytometric analysis of H22 cells after co-incubation with PBS, VNP, and T-5ApCB respectively at 37 °C for 1 h. Flow plots are representative of three independent biological samples. c Digital photos of mice at day 12 after treatment. Tumors were marked in red circles. H22 tumor-bearing mice (inoculated with 1 × 10⁶ carcinoma cells) were randomly divided into three groups and intravenously injected with PBS and 5 × 10⁵ CFU of VNP or T-5ApCB upon tumor size reaching ∼100 mm³ (defined as day 0). d Relative tumor growth after different treatments (n = 5). Data are presented as mean values ± SD. Significance was assessed using Student’s t test (two-tailed), giving p values: 0.000002 for T-5ApCB vs PBS; 0.0052 for T-5ApCB vs VNP. e Survival curves of H22 tumor-bearing mice after different treatments (n = 7). Data are presented as mean values ± SD. Significance was assessed using Student’s t test (two-tailed), giving p values: p < 0.0001 for T-5ApCB vs PBS; 0.0017 for T-5ApCB vs VNP. f Variation of bodyweight during treatment. **p < 0.01, ****p < 0.0001. Source data are provided as a Source Data file.