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. 2021 Nov 2;15:763856. doi: 10.3389/fnins.2021.763856

FIGURE 2.

FIGURE 2

Schematic of Irx3 and Irx5 expression in the mediobasal hypothalamus. (A) RNA in situ hybridization of Irx3 and Irx5 the mediobasal hypothalamus at postnatal day (P) 14. Irx3 and Irx5 expression is mainly found in radial glia-like cells lining the third ventricle, including RGL-NSCs, tanycytes, and ependymocytes. Panels from bottom to top (ventral to dorsal) show representative regions from: β-tanycytes (i), α-tanycytes (ii) and ependymocytes (iii). (B) Schematic of the mediobasal hypothalamus. Along the third ventricle, ependymocytes are located at the dorsal ventricular walls, followed by α-tanycytes, and β-tanycytes. Irx3- and Irx5-expressing RGL-NSCs are interspersed among α-tanycytes and β-tanycytes in the dorsal ventricular wall and floor.