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. 2021 Nov 2;15:768262. doi: 10.3389/fnins.2021.768262

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Copyright © 2021 Wang, Zhang, Zhu, Liang, Zuo, Ju, Song, Li, Hu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Photobiomodulation alleviated the neurotoxic effect of A1 astrocytes on DRG neurons. (A) DRG neurons treated with ACM collected 24 h after different treatments. Con, medium from control resting astrocytes; Con + PBM: medium from resting astrocytes treated with PBM; A1CM, medium from A1 astrocytes; PBM + A1CM, medium from A1 astrocytes treated with PBM. Representative immunofluorescence images of NeuN (red) and β III tubulin (green) in DRG neurons. Scale bar: 100 mm. (B,C) The length of axons in a single neuron and the number of neurons per field were analyzed. Five visual fields from each sample were randomly selected and analyzed. The experiments were independently repeated three times. *p < 0.01; ns, not significant.