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Primer sequences of PTEN, PI3K, and AKt.
| PCR primers | Sequences | |
|---|---|---|
| PTEN | Forward: | 5’-TGGATTCGACTTAGACTTGACCT-3’ |
| Reverse: | 5’-GGTGGGTTATGGTCTTCAAAAGG-3’ | |
| PI3K | Forward: | 5’-TATTTGGACTTTGCGACAAGACT-3’ |
| Reverse: | 5’-TCGAACGTACTGGTCTGGATAG-3’ | |
| AKt | Forward: | 5’-AGCGACGTGGCTATTGTGAAG-3’ |
| Reverse: | 5’-GCCATCATTCTTGAGGAGGAAGT-3’ | |
| GAPDH | Forward: | 5’-CTTAGCACCCCTGGCCAAG-3’ |
| Reverse: | 5’-GATGTTCTGGAGAGCCCCG-3’ | |
