Figure 4.

Fluorescence analysis demonstrated the spatial co-localization of infiltrated macrophages, exogenous omentin-1 (labeled by flag tag), and integrin receptors in the atherosclerotic plaque of ApoE^−/− mice. The aortic root sections were stained by primary antibodies against F4/80 (macrophage marker), αvβ3, αvβ5, and flag tag (the marker of exogenous omentin-1). Fluorescence secondary antibodies against protein from mice, rabbits, and rats were used to mark the localization of the primary antibodies. Three biological replicates were made in this analysis. (A) The macrophage (F4/80) was dyed red and αvβ3 was dyed green. The plaque area was marked by a dotted line. (B) The macrophage (F4/80) was dyed red and αvβ5 was dyed yellow. The plaque area was marked by a dotted line. (C) Flag-tagged exogenous omentin-1 (flag tag) was dyed red and αvβ3 was dyed green. The plaque area was marked by a dotted line. (D) Flag-tagged exogenous omentin-1 (flag tag) was dyed red and αvβ5 was dyed yellow. The plaque area was marked by a dotted line. (E) Flag-tagged exogenous omentin-1 (flag tag) was dyed red and macrophage (F4/80) was dyed green. The plaque area was marked by a dotted line (Lu = the lumen of the aorta).