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. 2021 Nov 2;12:719504. doi: 10.3389/fpls.2021.719504

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Copyright © 2021 Ijaz, Mumtaz, Wang, Ahmad, Saqib, Maqbool, Zaheer, Wang and Mustafa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Demonstration of minerals solubilization; phosphorus (P) by ASH11 (A) and ASH19 (B), zinc (Zn) by ASH7 (C) and ASH22 (D), and potassium (K) by ASH10 (E) and ASH22 (F); manganese solubilizing bacterial (MSB) strains were spot inoculated on Pikovskaya agar amended with tri-calcium phosphate and tris-minimal salt agar amended with zinc oxide for P and Zn solubilization; K solubilization was determined by inoculating MSB strains on mica-amended Aleksandrov agar media; these solubilization assays were incubated for 7 days and appearance of solubilization zones were considered positive for respective mineral solubilization; K solubilization zones were observed through diluting agar media with dilute iodine solution for 10 min; PSB, P solubilizing bacteria; ZSB, Zn solubilizing bacteria; KSB, K solubilizing bacteria.