. 2021 Nov 2;12:719504. doi: 10.3389/fpls.2021.719504

TABLE 2.

Qualitative and quantitative solubilization of phosphorus by manganese solubilizing bacterial strains isolated from maize rhizosphere.

Strains	PSD (mm)	BGD (mm)	PSI	PSE (%)	SPC (μg mL^–1)
Control	ND	ND	ND	ND	0.43 ± 0.02^e
ASH4	13.67 ± 0.27^de	9.33 ± 0.27^f	2.47 ± 0.03^c	147.16 ± 3.78^c	1.67 ± 0.12^cd
ASH6	10.00 ± 0.09^f	4.33 ± 0.27^g	3.33 ± 0.10^a	232.93 ± 13.61^a	2.27 ± 0.24^c
ASH7	11.33 ± 0.54^ef	6.33 ± 0.54^g	2.80 ± 0.02^b	180.95 ± 7.77^b	1.93 ± 0.26^cd
ASH8	14.00 ± 0.94^d	6.65 ± 0.54^g	3.11 ± 0.07^a	211.11 ± 9.07^a	2.50 ± 0.79^c
ASH9	18.66 ± 0.27^c	12.67 ± 0.27^de	2.47 ± 0.01^c	147.44 ± 1.04^c	1.80 ± 0.32^cd
ASH10	12.10 ± 0.47^def	6.65 ± 0.27^g	2.82 ± 0.12^b	181.75 ± 14.64^b	0.63 ± 0.05^d
ASH11	11.33 ± 1.08^ef	5.00 ± 0.47^g	3.28 ± 0.11^a	227.78 ± 12.02^a	1.83 ± 0.21^cd
ASH12	23.00 ± 0.81^b	15.66 ± 1.18^bc	2.48 ± 0.05^c	148.29 ± 6.68^c	6.67 ± 0.69^a
ASH17	25.67 ± 0.72^a	21.67 ± 0.72^a	2.19 ± 0.07^d	118.12 ± 0.63^d	2.50 ± 0.41^c
ASH19	22.33 ± 1.18^b	18.00 ± 0.94^b	2.24 ± 0.09^cd	124.07 ± 0.75^cd	1.83 ± 0.59^cd
ASH20	21.67 ± 0.72^b	17.33 ± 0.98^bc	2.25 ± 0.06^cd	125.54 ± 3.19^cd	0.73 ± 0.09^d
ASH22	18.00 ± 0.47^c	15.00 ± 0.81^cd	2.21 ± 0.04^cd	120.07 ± 4.37^cd	4.26 ± 0.07^b
ASH24	14.00 ± 0.47^d	11.33 ± 0.54^ef	2.24 ± 0.03^cd	122.89 ± 3.17^cd	0.80 ± 0.08^d
LSD	1.21	1.16	0.14	13.48	0.67

Manganese solubilizing bacterial (MSB) strains were screened for qualitative solubilization of phosphorus (P) by spot inoculating bacterial biomass on Pikovskaya (PVK) agar media; P solubilization diameter (PSD) and bacterial growth diameter (BGD) were recorded after 7 days of incubation; the P solubilization index (PSI) and P solubilization efficiency (PSE) were calculated through using PSD and BGD; the quantitative P solubilization was determined by inoculating PVK broth for 7 days and solubilized P concentration (SPC) was detected through a colorimetric method by using a spectrophotometer; data presented are the mean of three replications ± standard error and least significant difference (LSD) test was performed at 5% (p ≤ 0.05) probability level; the means in a vertical line for each attributes sharing common letters were considered statistically similar to each other; ND, not detected.