TABLE 3.
Qualitative and quantitative solubilization of zinc by manganese solubilizing bacterial strains isolated from maize rhizosphere.
| Strains | ZSD (mm) | BGD (mm) | ZSI | ZSE (%) | SZC (μg mL–1) |
| Control | ND | ND | ND | ND | 0.12 ± 0.01f |
| ASH4 | 28.50 ± 1.76a | 19.00 ± 2.12a | 3.52 ± 0.13cde | 151.37 ± 7.63cde | NT |
| ASH6 | 18.50 ± 0.03cde | 10.00 ± 0.70bc | 2.86 ± 0.02cd | 185.86 ± 9.64cd | 16.54 ± 0.14d |
| ASH7 | 22.00 ± 1.41bc | 11.50 ± 1.06bc | 2.92 ± 0.05c | 192.13 ± 5.43c | NT |
| ASH8 | 14.50 ± 0.35fgh | 4.00 ± 0.01e | 4.63 ± 0.08ab | 361.80 ± 8.83ab | NT |
| ASH9 | 13.00 ± 1.41gh | 9.00 ± 0.70cd | 2.44 ± 0.11cde | 143.15 ± 4.41cde | NT |
| ASH10 | 20.00 ± 0.65bcd | 12.00 ± 0.02bc | 2.67 ± 0.40cde | 166.36 ± 0.02cde | NT |
| ASH11 | 12.50 ± 0.35h | 4.00 ± 0.01e | 4.13 ± 0.08b | 311.90 ± 8.83b | 17.86 ± 0.25c |
| ASH12 | 16.00 ± 0.01e–h | 12.50 ± 0.35bc | 2.28 ± 0.06e | 128.02 ± 3.62e | NT |
| ASH17 | 17.45 ± 0.35def | 13.00 ± 0.70b | 2.35 ± 0.02de | 135.42 ± 4.62de | NT |
| ASH19 | 16.50 ± 0.35d–g | 4.50 ± 0.35e | 4.73 ± 0.33a | 372.30 ± 37.12a | 23.17 ± 0.21a |
| ASH20 | 22.20 ± 0.35b | 12.00 ± 0.70bc | 2.88 ± 0.01cd | 188.14 ± 8.15cd | 12.20 ± 0.31e |
| ASH22 | 8.50 ± 0.35i | 6.00 ± 0.04de | 2.42 ± 0.05cde | 141.07 ± 5.89cde | 22.05 ± 0.19b |
| LSD | 1.63 | 1.62 | 0.25 | 24.97 | 0.42 |
Manganese solubilizing bacterial (MSB) strains were screened for qualitative solubilization of zinc (Zn) by spot inoculating bacterial biomass on ZnO2-amended tris-minimal salt agar media. Zn solubilization diameter (ZSD) and bacterial growth diameter (BGD) were recorded after 7 days of incubation; the Zn solubilization index (ZSI) and Zn solubilization efficiency (ZSE) were calculated through using ZSD and BGD; the quantitative Zn solubilization was determined by inoculating the ZnO2-amended tris-minimal salt broth for 7 days and solubilized Zn concentration (SZC) was detected through inductively coupled plasma optical emission spectroscopy; data presented here are the mean of three replications ± standard error and least significant difference (LSD) test was performed at 5% (p ≤ 0.05) probability level; the means in a vertical line for each attributes sharing common letters were considered statistically similar to each other; NT, not tested.