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. 2021 Nov 2;8:738060. doi: 10.3389/fcvm.2021.738060

Figure 3.

Figure 3

Atherosclerosis is attenuated in LDLR−/− hamsters treated with ezetimibe after 40 days of HFD. Representative pictures of histochemistry sections of the aortic root (A) and en face analysis of the whole length of the aorta (B) by oil red O staining (n = 10). Scale bars, 500 μm. Total absolute oil red O positive area in all sections of the aortic root (B) and relative oil red O-positive area of the aorta en face (n = 10). Data are shown as mean ± SEM. *p < 0.05, **p < 0.01 compared with the HFD group, where the one-way ANOVA with Tukey's multiple comparisons test was performed. (C) Representative images of H&E staining of sections of the aortic arch. Scale bar = 200 or 50 μm, respectively. The expression of ICAM-1 (D) and VCAM-1 (E) in cultured HUVECs was analyzed by western blotting, the right panel was the quantitative bar chart analysis of the left panel. The serum from normal CD-fed WT hamsters was used as the control, the expression of ICAM-1 and VCAM-1 incubated with serum from HFD diet-fed hamsters was upregulated and downregulated with the serum from HFD-fed hamsters with ezetimibe administration. Data are shown as mean ± SEM. Two-tailed unpaired t-test, # HFD+EZE vs. HFD, #p < 0.05, and the one-way ANOVA with Tukey's multiple comparisons test was performed.