TABLE 3.
Overproduction of Red1 or Mek1 promotes sporulation of mutants that arrest at the pachytene checkpoint
| Strain | Mutant genotype | Sporulationa (%) | Nuclear divisiona (%) |
|---|---|---|---|
| JM156 | zip2 + YEp351b | 9.4 | 9.4 |
| JM158 | zip2 + pJ17 (YEp351 RED1) | 66.1 | 64.2 |
| JM157 | zip2 + pJ16 (YEp351 MEK1) | 32.0 | 27.6 |
| JM159 | dmc1 + YEp351 | 29.8 | 31.2 |
| JM161 | dmc1 + pJ17 (YEp351 RED1) | 29.8 | 28.0 |
| JM160 | dmc1 + pJ16 (YEp351 MEK1) | 30.1 | 33.0 |
| JM541 | hop2 + YEp352 | 0 | 0 |
| JM543 | hop2 + pB86 (YEp352 RED1) | 20.0 | 19.2 |
| JM542 | hop2 + pB133 (YEp352 MEK1) | 6.6 | 7.0 |
a
Measured after 72 h in sporulation medium.
b
Strain JM156 is the control for JM158 and JM157, strain JM159 is the control for JM161 and JM160, and strain JM541 is the control for JM543 and JM542.