TABLE 4.
Overproduction of Red1 decreases gene conversion
| Strain | Mutant genotype | Prototrophsa
|
||
|---|---|---|---|---|
| Histidine | Threonine | Tryptophan | ||
| JM117 | spo13 + YEp352c | 41.0 | 2.2 | 1.9 |
| JM116 | spo13 + pB86 (YEp352 RED1) | 22.0 (1.8)b | 0.9 (2.4) | 1.0 (1.9) |
| JM115 | spo13 + pB133 (YEp352 MEK1) | 39.0 (1.0) | 2.2 (1.0) | 1.8 (1.0) |
| JM119 | spo13 + YEp24 | 37.0 | 2.4 | 2.2 |
| JM118 | spo13 + pNH83-2 (YEp24 HOP1) | 28.0 (1.3) | 1.6 (1.5) | 1.5 (1.5) |
| JM112 | spo13 zip1 + YEp352 | 36.4 | 1.7 | 6.1 |
| JM111 | spo13 zip1 + pB86 (YEp352 RED1) | 6.2 (5.6) | 0.4 (3.6) | 1.5 (4.7) |
| JM110 | spo13 zip1 + pB133 (YEp352 MEK1) | 35.5 (1.0) | 1.6 (1.1) | 4.3 (1.4) |
| JM212 | spo13 zip1 + pJ14 (YEp352 MEK1 RED1) | 25.2 (1.4) | 1.1 (1.5) | 3.8 (1.6) |
| JM213 | spo13 zip1 + pJ70 (YEp352 MEK1 HOP1) | 38.6 (0.94) | 1.3 (1.3) | 4.4 (1.4) |
| JM214 | spo13 zip1 + pJ66 (YEp352 RED1 HOP1) | 31.3 (1.2) | 1.3 (1.3) | 4.7 (1.3) |
| JM113 | spo13 zip1 + YEp24 | 35.0 | 1.7 | 7.0 |
| JM114 | spo13 zip1 + pNH83-2 (YEp24 HOP1) | 23.3 (1.5) | 1.2 (1.4) | 4.6 (1.5) |
a
Cells returned to growth after 72 h in sporulation medium. Values indicated are frequencies per 104 cells.
b
Values in parentheses indicate the fold decrease relative to that of the appropriate control strain.
c
Strain JM117 is the control for JM116 and JM115; strain JM119 is the control for JM118; strain JM112 is the control for JM111, JM110, JM212, JM213, and JM214; and strain JM113 is the control for JM114.