TABLE 5.
Red1 overproduction reduces crossing over in a zip1 mutant
| Strain | Mutant genotype | HIS4 LEU2 (cM)a | LEU2 MAT (cM)a | Spore viability (%)a |
|---|---|---|---|---|
| BS395 | spo13 + YEplac112 | 43.0 | 31.0 | 79.0 |
| BS396 | spo13 + pAV62 (YEplac112 RED1) | 19.2 (2.2)b | 17.3 (1.8) | 69.0 |
| JM289 | spo13 + pJ104 (YEplac112 MEK1) | 46.2 (0.93) | 32.9 (0.94) | 76.0 |
| BS398 | spo13 zip1 + YEplac112 | 24.8 | 19.0 | 44.1 |
| BS399 | spo13 zip1 + pAV62 (YEplac112 RED1) | 9.5 (2.6) | 7.1 (2.7) | 19.0 |
| JM290 | spo13 zip1 + pJ104 (YEplac112 MEK1) | 20.9 (1.2) | 16.5 (1.2) | 37.5 |
a
At least 100 dyads were analyzed per strain.
b
Strain BS395 is the control for BS396 and JM289; strain BS398 is the control for BS399 and JM290.