Figure 3.

Surface plasmon resonance (SPR) characterization of native and fusion GDHγα

(A–E) Single cycle kinetics analysis by SPR of the direct binding of (A) native GDHγα, (B) GDH γα-L_GBP, (C) GDHγα-M_GBP, (D) GDHγα-T_GBP, or (E) GDHγα-V_GBP to gold substrate. (A–E) Experimental data are shown in black; calculated fits using a 1:1 binding model with a global fitting on all injected concentrations are shown in red. Injections of proteins for the association phase are indicated by the solid arrows, and injection of pure PBS buffer for dissociation phase are indicated by dashed arrows. Protein concentrations injected were 6.25, 12.5, 25, 50, and 100 nM for all samples.

(F) Comparison of titration curve obtained by plotting binding responses against protein concentration.