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. 2021 Oct 29;24(11):103368. doi: 10.1016/j.isci.2021.103368

Figure 3.

Figure 3

Depletion of Y14 impairs polyploidization

(A) HEL cells were transduced with lentivirus expressing shY14 (+) or control shRNA (−) for 48 h, followed by mock-treated or PMA (5 nM) treatment for 3 days. RT-qPCR of CD41 and CD61 and immunoblotting (IB) of Y14 and GAPDH were performed; experiments were performed in triplicate (mean ± SEM; ∗p < 0.05, ∗∗p < 0.01).

(B) DNA histogram analysis of HEL cells as in panel A was performed. The stacked-column bar shows ploidy distribution; a peak between 2N and 4N (arrow) was quantified as 2N.

(C) HEL cells were transduced and treated as in panel (A). Immunoblotting was performed using antibodies as indicated. The relative expression level of phospho-H3 (pH3) and CDC20 was indicated; average was obtained from 3 times of the experiments.