REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-Y14	Abcam	Cat# ab5828; RRID: AB_2269494
Mouse monoclonal anti-phospho-Histone H2A.X (gH2AX)	Sigma-Aldrich	Cat# 05-636
Mouse monoclonal anti-beta actin	Proteintech	Cat# 60008-1-Ig; RRID: AB_2289225
Mouse monoclonal anti-GAPDH	Proteintech	Cat# 60004-1-Ig; RRID: AB_2107436
Mouse monoclonal anti-cyclin B1	BD Pharmingen	Cat# 557149
Rabbit monoclonal anti-phospho-Histone H3	Cell Signaling Technology	Cat# 3377; RRID: AB_1549592
Rabbit monoclonal anti-Histone H3	Cell Signaling Technology	Cat# 4499; RRID: AB_10544537
Rabbit polyclonal anti-cyclin B2	ABclonal	Cat# A3351; RRID: AB_2765068
Rabbit polyclonal anti-vWF	Proteintech	Cat# 11778-1-AP; RRID: AB_10642840
Rabbit polyclonal anti-p53	Proteintech	Cat# 10442-1-AP; RRID: AB_2206609
Rabbit polyclonal anti-p21	Proteintech	Cat# 10355-1-AP; RRID: AB_2077682
Rabbit polyclonal anti-p21 (C-19)	Santa Cruz	Cat# sc-397; RRID: AB_632126
Rabbit polyclonal anti-CDK1	Proteintech	Cat# 19532-1-AP; RRID: AB_10638617
Rabbit polyclonal anti-Cdc20	Abcam	Cat# ab183479
Rabbit polyclonal anti-Cleaved Caspase-3 (Asp175)	Cell Signaling Technology	Cat# 9661; RRID: AB_2341188
Rabbit polyclonal anti-Y14	Bethyl	Cat# A301-033A; RRID: AB_2300943
Rabbit polyclonal anti-gamma H2AX (p Ser 139)	Novus Biologicals	Cat# NB100-384; RRID: AB_10002815
Rat Monoclonal anti-Mouse GP-VI	EMFRET Analytics	Cat# M011-1; RRID: AB_2827531
Rat Monoclonal anti-GP-IX	EMFRET Analytics	Cat# M051-1; RRID: AB_2827529
Rat anti-Mouse α2bβ3	EMFRET Analytics	Cat# M021-1
Rat Monoclonal anti-JON/A	EMFRET Analytics	Cat# M023-2; RRID: AB_2833084
Rabbit polyclonal anti-Fibrinogen	EMFRET Analytics	Cat# P140-1
Rabbit Polyclonal anti-vWF	EMFRET Analytics	Cat# P150-1
Rat anti-Mouse P-selectin	EMFRET Analytics	Cat# M130-1; RRID: AB_2890922
Rat anti-Mouse CD41	BD Biosciences	Cat# 553848; RRID: AB_395085
Rat anti-Mouse IgG	BD Biosciences	Cat# 554684; RRID: AB_395508
Mouse anti-Human CD41	BD Biosciences	Cat# 555466; RRID: AB_395858
Anti-Mouse IgG HRP	GE Healthcare	Cat# NA931; RRID: AB_772210
Anti-Rabbit IgG HRP	GE Healthcare	Cat# NA934; RRID: AB_772206
Bacterial and virus strains
Luc-targeting shRNA-expressing lentivirus	RNAi Core Facility, Academia Sinica	N/A
Y14-targeting shRNA-expressing lentivirus	RNAi Core Facility, Academia Sinica	N/A
P53-targeting shRNA-expressing lentivirus	RNAi Core Facility, Academia Sinica	N/A
Chemicals, peptides, and recombinant proteins
PMA	Sigma-Aldrich	Cat# P1585
Pifithrin-α	Sigma-Aldrich	Cat# 506132
PAR4 activating peptide	Bachem	Cat# H6054
Thrombopotein (TPO), Mouse	GenScript	Cat# Z03175
Critical commercial assays
Click-iTTM EdU Alexa FluorTM 647 Flow Cytometry Assay kit	ThermoFisher Scientific	Cat# C10340
Deposited data
RNA-seq data	This study	PRJNA684792
Experimental models: Organisms/strains
Mouse: Pf4-Cre; Rbm8af/f (C57BL/6J strain)	This study	N/A
Mouse: Pf4-Cre; Rbm8af/f; Trp53f/f (C57BL/6J strain)	This study	N/A
Oligonucleotides
All primers used in this study were listed in Table S4.	This study	N/A
Recombinant DNA
Plasmid: pcDNA-FLAG-p53-expression vector	(Lu et al., 2017)	N/A
Plasmid: pcDNA-FLAG-p53 M133K mutant expression vector	This study	N/A
Software and algorithms
ImageJ	Schneider et al., 2016	http://imagej.nih.gov/ij/
Prism	GraphPad	https://www.graphpad.com/scientific-software/prism/
Trimmomatic v.0.38	Bolger et al., 2014	http://www.usadellab.org/cms/?page=trimmomatic
HISAT2	Kim et al., 2015; Sahraeian et al., 2017	http://daehwankimlab.github.io/hisat2/
featureCounts	Liao et al., 2014	N/A
DESeq2	Love et al., 2014	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
clusterProfiler (3.10.1)	Subramanian et al., 2005	https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html
Other
Mendeley data	This study	https://doi.org/10.17632/ycpm9nyfr2.1