Figure 2.

UC colonoids have defects in mucus secretion and barrier function.(A) Methanol–Carnoy’s fixed DF colonoid monolayers stained with MUC2 (green), nucleus (blue). Representative confocal XZ (above) and 3D-XYZ (below) projections depicting the MUC2 layer in colonoids monolayer is shown. (B) Average number of GCs expressed after 5 days of differentiation of colonoid monolayers. (C) Differences in mRNA expression of C2GNT2 mRNA after differentiation of monolayers from HS and inactive and active UC sites. (D) Bacterial 16S rRNA expression in colonoids after 8-hour infection of DF monolayers. (A) and (B) Multiple areas of monolayers from each subject were analyzed. (C) and (D) n = 3 monolayers from each group were analyzed at different times. Results are shown as mean ± SEM. ∗P < .05 vs HS. Scale bar = 20 μm.