Figure 7.

TNF-α (5 ng/mL, 5 days) treatment decreases GC number.(A) Monolayers from HS and UC inactive and active sites were differentiated alone or with TNF-α (5 ng/mL) for 5 days, and MUC2 positive GCs (green) were analyzed using confocal imaging. TNF-α (5 ng/mL) was refreshed during medium change at second day of 5-day period. (B) Average number of GCs expressed in untreated or TNF-α treated monolayers. Results are shown as means ± SEMs.∗P < .05 vs control/untreated monolayers, n = 3 separate monolayer from each group. Scale bar = 20 μm.