Figure 3.

Cytokine production by innate-like T cells and T cells in mice treated with i6-FP. IFN-γ, TNF-α, and IL-17A intracellular staining of MAIT cells, iNKT cells, γδ T cells, and CD4⁺ and CD8⁺ T cells in splenocytes from mice in the control group (n = 17) or i6-FP-treated mice (n = 18) 6 hours after phorbol 12-myristate 13-acetate (PMA) and ionomycin stimulation are shown. Representative flow cytometry profiles (A) and the percentages of indicated cytokine-producing cells (B). Data are represented as the mean ± standard error of the mean in each group. Significance was determined using Welch’s t-test. ∗P < .05, ∗∗P < .01. ∗∗∗P < .001. Each symbol represents data from an individual mouse.